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KMID : 0374019830060010035
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Ewha Medical Journal
1983 Volume.6 No. 1 p.35 ~ p.46
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Effects of Chronic Alcohol Feeding on the Lipid Contents in the Rat Liver and the Hepatic Microsomal 2-Acetylaminofluorene (2-AAF) Hydroxylation in Vitro
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Abstract
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It is now well established that ethanol excerts different effects on hepatic cellular metabolism, depending mainly on the duration of its intake. And induction of quantitative and qualitative alterations in the cytochrome P-450 of hepatic microsomes _after chronic ethanol feeding is a well -documented- phenomenon.
But the effect of ethanol ingestion on the drug metabolism appear paradoxic and the ability of chronic ethanol ingestion to potentiate the carcinogenicity of 2-acetylaminofluorene (2-AAF) by microsomal activation is not confirmed yet.
The present study was undertaken to demonstrate the effects of 150/0, 30% ethanol and a commercial liquor feeding for 16 weeks on the lipids in the rat liver, hepatic microsomal cytochrome ´P-450 and b5, and to elucidate whether or not ethanol-induced rat - rnicrosomes can increase the risk of cancer by increasing the rate of metabolic activation of 2-AAF in vitro.
The results are followings.
1) The chronic alcohol feeding increased the contents of total cholesterol and triglyceride in the liver, but did not make a significant influence on the phospholipid contents of liver.
2) The 8 week alcohol feeding increased the hepatic microsomal cytochrome P-
450 without the change. of cytochrome b5 but the 16 week feeding decreased P-450 Y J
significantly. ´
3) The chronic alcohol feeding elevated the AAF N-hydroxylation continuously but did not influence on AAF ring-hydroxylation.
4) The correlation of AAF N-hydroxylation and the increases of hepatic lipid contents was indicated.
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